700160  Myeloperoxidase Peroxidation Assay Kit

MPO Peroxidation Assay Kit



• See below for pricing and ordering
• See more Enzyme Activity; Fluorescence; Assay Kits; Cardiology; Inflammation

Let Cayman analyze your samples for you. See EIA Service for details and availability.

For active pharmaceutical ingredients (APIs) related to Myeloperoxidase Peroxidation Assay Kit, see Cayman Pharma

Myeloperoxidase (MPO) is a member of the heme peroxidase superfamily and is stored within the azurophilic granules of leukocytes.1 MPO is found within circulating neutrophils, monocytes, and some tissue macrophages.2 A unique activity of MPO is its ability to use chloride as a cosubstrate with hydrogen peroxide to generate chlorinating oxidants such as hypochlorous acid, a potent antimicrobial agent.3 Recently, evidence has emerged that MPO-derived oxidants contribute to tissue damage and the initiation and propagation of acute and chronic vascular inflammatory diseases.4,5 The fact that circulating levels of MPO have been shown to predict risks for major adverse cardiac events and that levels of MPO-derived chlorinated compounds are specific biomarkers for disease progression, has attracted considerable interest in the development of therapeutically useful MPO inhibitors.6 MPO also oxidizes a variety of substrates, including phenols and anilines, via the classic peroxidation cycle. The relative concentrations of chloride and the reducing substrate determine whether MPO uses hydrogen peroxide for chlorination or peroxidation. Cayman’s MPO Peroxidation Assay provides a convenient fluorescence-based method for detecting the MPO peroxidase activity in both crude cell lysates and purified enzyme preparations. The assay utilizes the peroxidase component of MPO. The reaction between hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence can be easily analyzed with an excitation wavelength of 530-540 nm and emission wavelength of 585-595 nm. The kit includes a MPO-specific inhibitor for distinguishing between MPO activity from MPO-independent fluorescence.
1  Yamada, M., Kurahashi, K. Regulation of myeloperoxidase gene expression during differentiation of human myeloid leukemia HL-60 cells. J Biol Chem 259(5) 3021-3025 (1984).
2  Schultz, J., Kaminker, K. Myeloperoxidase of the leucocyte of normal human blood.1 I. Content and localization. Arch Biochem Biophys 96 465-467 (1962).
3  Harrison, J.E., Schultz, J. Studies on the chlorinating activity of myeloperoxidase. J Biol Chem 251(5) 1371-1374 (1976).
4  Podrez, E.A., Abu-Soud, H.M., Hazen, S.L. Myeloperoxidase-generated oxidants and atherosclerosis. Free Radic Biol Med 28(12) 1717-1725 (2000).
5  Zhang, R., Brennan, M., Fu, X., et al. Association between myeloperoxidase levels and risk of coronary artery disease. JAMA 286(17) 2136-2142 (2001).
6  Malle, E., Furtmüller, P.G., Sattler, W., et al. Myeloperoxidase: A target for new drug development?. Br J Pharmacol 152 838-854 (2007).

Download Kit Booklet 557 Kb PDF
Download Material Safety Data Sheet (MSDS) 137 Kb PDF

Pricing Information

Prices listed here are the Manufacturer's Suggested European Retail Price. The actual prices quoted by each individual distributor may vary. Please visit the Global Buyer's Guide on the Cayman Chemical (world) website to locate a distributor in your region.

This product is also available to buy in bulk quantities. Please contact us for a quote or to purchase.

Cayman strives to be a reliable biochemical reagent vendor by providing the best possible products and services. To ask for assistance with one of our products please contact our Technical Help staff.

Warning This product is not for human or veterinary use.

Item total €0.00
Shopping cart total €0.00
Size Price Quantity
2 x 96 wells €258.00
Pricing updated 2010-08-01.
Prices are subject to change without notice.