600170  Aldehyde Site (DNA and Protein) Detection Kit



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• See more Cell-Based Assay; Assay Kits; Oxidative Injury

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Oxidative damage occurs in all living organisms from reactive oxygen species (ROS), which are a consequence of normal body processes such as metabolism. ROS react with proteins, resulting in protein modification, such as introduction of carbonyl groups into the protein. The modified proteins are dysfunctional and can be removed through degradation. Both mitochondrial DNA and nuclear DNA are constantly exposed to oxygen radicals, causing extensive oxidative damage. DNA damage by ROS has significant consequences since it causes mutations and genomic instability. Studies have shown that oxidative DNA damage accumulates with aging. Oxidative DNA damage has been implicated to be important in many diseases, including cancer.1 Assessment of this damage in various biological matrices is essential for understanding the mechanisms of oxidative damage and its biological effects. Over the past decades, many analytical techniques, such as LC- and GC-mass spectrometry, have been developed to measure oxidative DNA base damage. More recently, antibody-based immunoassays, assays involving the use of DNA repair glycosylases, such as the comet assay, and slot blot assays using an aldehyde reactive probe (ARP), such as O-(biotinylcarbazoylmethyl) hydroxylamine, have been used to assess oxidative DNA damage.2,3 The ARP probe reacts specifically with aldehyde groups that result from protein or DNA modification. By using an excess amount of ARP, aldehyde sites in both protein and DNA can be converted to biotin-tagged aldehyde sites, which can be detected using avidin-conjugated reporters. Cayman’s Aldehyde Site (DNA and Protein) Detection Kit employs an ARP, O-(biotinylcarbazoylmethyl) hydroxylamine, as a probe to detect aldehyde sites in cells. Epigallocatechin Gallate (EGCG), a compound known to react with culture medium to generate hydrogen peroxide (H2O2) and cause DNA damage,4,5 is included to be used as a positive control. The kit is easy to use and can be easily adapted to high throughput screening for compounds imposing oxidative stress on cells or organisms.
1  Cooke, M.S., Evans, M.D., Dizdaroglu, M., et al. Oxidative DNA damage: Mechanisms, mutation, and disease. FASEB J 17 1195-1214 (2003).
2  Nakamura, J., Walker, V.E., Upton, P.B., et al. Highly sensitive apurinic/apyrimidinic site assay can detect spontaneous and chemically induced depurination under physiological conditions. Cancer Res 58 222-225 (1998).
3  Poulsen, H.E., Weimann, A., Loft, S. Methods to detect DNA damage by free radicals: Relation to excercise. Proc Nutr Soc 58 1007-1014 (1999).
4  Elbling, L., Weiss, R., Teufelhofer, O., et al. Green tea extract and (−)-epigallocatechin-3-gallate, the major tea catechin, exert oxidant but lack antioxidant activities. FASEB J 19(7) 807-809 (2005).
5  Tachibana, H. Molecular basis for cancer chemoprevention by green tea polyphenol EGCG. Forum Nutr 61 156-169 (2009).

Download Kit Booklet 798 Kb PDF
Download Material Safety Data Sheet (MSDS) 86 Kb PDF

Pricing Information

Prices listed here are the Manufacturer's Suggested European Retail Price. The actual prices quoted by each individual distributor may vary. Please visit the Global Buyer's Guide on the Cayman Chemical (world) website to locate a distributor in your region.

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96 wells €300.00
Pricing updated 2010-08-01.
Prices are subject to change without notice.