583301  Interleukin-1α (human) EIA Kit

Interleukin-1α (human) ELISA Kit; IL-1α (human) EIA Kit

You may be eligible to receive a free sample Interleukin-1α (human) EIA Kit under the Cayman Challenge program.

Cayman offers free EIA analysis tools you may find helpful.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

For active pharmaceutical ingredients (APIs) related to Interleukin-1α (human) EIA Kit, see Cayman Pharma

Limit of detection: 31.3 pg/ml · Interleukin-1 (IL-1) is a name for a family of proteins which includes IL-α, IL-1β, ILra (interleukin receptor antagonist) and IL-18. IL-1α is synthesized as a 33 kDa (271 amino acid) pro-cytokine that is enzymatically cleaved by calpain into the active 159 amino acid, 17 kDa peptide.1,2,3 IL-1α is produced by many cells types including macrophages, monocytes, astrocytes, keratinocytes, adipocytes, T-cells, and eosinophils.4 Although IL-1α and IL-1β exhibit only 26% amino acid identity,1 they bind to the same cell-surface receptors, IL-1 RI and IL-1 RII, present on a variety of cell types involved in immune or inflammatory responses.5,6,7 Normal production of IL-1 is critical for mediating host responses to infection and injury.8,9 Disease states in which IL-1 actively participates include inflammatory diseases such as arthritis, inflammatory bowel disease, and shock, as well as atherosclerosis, allergic disease, and some types of cancer.8,9 Cayman’s IL-1α (human) EIA Kit is an immunometric (i.e., sandwich) EIA that permits IL-1α measurements within the range of 0-250 pg/ml, typically with a limit of detection of <31.3 pg/ml.

1  March, C.J., Mosley, B., Larsen, A., et al. Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature 315 641-647 (1985). 2  Kobayashi, Y., Yamamoto, K., Saido, T., et al. Identification of calcium-activated neutral protease as a processing enzyme of human interleukin 1α Proc Natl Acad Sci USA 87, 5548-5552 (1990). 3  Watanabe, N., Kobayashi, Y. Selective release of a processed form of interleukin 1α Cytokine 6(6), 597-601 (1994). 4  Dinarello, C.A. Interleukin-1 and its biologically related cytokines. Adv Immunol 44 153-205 (1989). 5  Sims, J.E., March, C.J., Cosman, D., et al. cDNA expression cloning of the IL-1 receptor, a member of the immunoglobulin superfamily. Science 241 585-589 (1988). 6  Sims, J.E., Acres, R.B., Grubin, C.E., et al. Cloning the interleukin 1 receptor from human T cells. Proc Natl Acad Sci USA 86 8946-8950 (1989). 7  McMahan, C.J., Slack, J.L., Mosley, B., et al. A novel IL-1 receptor, cloned from B cells by mammaliam expression, is expressed in many cell types. EMBO J 10(10) 2821-2832 (1991). 8  Dinarello, C.A. Interleukin-1 and the pathogenesis of the acute-phase response. N Engl J Med 311 1413-1418 (1984). 9  Rosenwasser, L.J. Biologic activities of IL-1 and its role in human disease. J Allergy Clin Immunol 102 344-350 (1998).

	Download Kit Booklet 762 Kb PDF
	Download Abbreviated Kit Protocol 416 Kb PDF
	Download Material Safety Data Sheet (MSDS) 262 Kb PDF

Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.