534721  15(S)-HETE EIA Kit

15(S)-HETE ELISA Kit

You may be eligible to receive a free sample 15(S)-HETE EIA Kit under the Cayman Challenge program.

Cayman offers free EIA analysis tools you may find helpful.

Let Cayman analyze your samples for you. See EIA Service for details and availability.

For active pharmaceutical ingredients (APIs) related to 15(S)-HETE EIA Kit, see Cayman Pharma

Limit of detection: 80% B/B0: 170 pg/ml · Sensitivity: 50% B/B0: 960 pg/ml · 15(S)-HETE is produced from arachidonic acid by the enzyme 15-lipoxygenase (15-LO). In humans it is formed primarily in the respiratory epithelium, leukocytes, and reticulocytes.1 15(S)-HETE has been detected in high concentrations in nasal secretions and may contribute to allergic rhinitis.2 15(S)-HETE has anti-inflammatory properties, inhibiting carragheenan-induced arthritis and lowering leukotriene B4 (LTB4) concentrations in the synovial fluid of dogs.3 It may regulate T-lymphocytes by inhibiting 5- and 12-LOs.4 It is also a vasoconstrictor, constricting cerebral and coronary arteries of dogs in vitro and cerebral arteries of pigs in vivo.5 15(S)-HETE may also play a role in cancer, inhibiting apoptosis by carcinosarcoma cells.6 Our 15(S)-HETE EIA is a competitive assay that permits the measurement of 15(S)-HETE within the range of 10-0.1 ng/ml, typically with a detection limit of 0.2 ng/ml. This assay is very specific for 15(S)-HETE, showing very low crossreactivity to other HETEs.

1  Hunter, J.A., Finkbeiner, W.E., Nadel, J.A., et al. Predominant generation of 15-lipoxygenase metabolites of arachidonic acid by epithelial cells from human trachea. Proc Natl Acad Sci USA 82 4633-4637 (1985). 2  Ramis, I., Roselló-Catafau, J., Bulbena, O., et al. 15-Hydroxyeicosatetraenoic acid as a major eicosanoid in nasal secretions: Assay by high-performance liquid chromatographic-radioimmunoassay and gas chromatographic-mass spectrometric procedures. J Chromatogr 496 416-422 (1989). 3  Fogh, K., Stender Hansen, E., Herlin, T., et al. 15-Hydroxy-eicosatetraenoic acid, (15-HETE) inhibits carragheenan-induced experimental arthritis and reduces synovial fluid leukotriene B4 (LTB4). Prostaglandins 37 213-228 (1989). 4  Bailey, J.M., Bryant, R.W., Low, C.E., et al. Regulation of T-lymphocyte mitogenesis by the leukocyte product 15-hydroxy-eicosatetraenoic acid (15-HETE). Cell Immunol 67 112-120 (1982). 5  Lovelady, G.K., Mirro, R., Armstead, W.M., et al. Effect of 15-HETE on cerebral arterioles of newborn pigs. Prostaglandins 36 507-513 (1988). 6  Tang, D.G., Chen, Y.Q., Honn, K.V. Arachidonate lipoxygenases as essential regulators of cell survival and apoptosis. Proc Natl Acad Sci USA 93 5241-5246 (1996).

	Download Kit Booklet 653 Kb PDF
	Download Abbreviated Kit Protocol 424 Kb PDF
	Download Material Safety Data Sheet (MSDS) 176 Kb PDF

Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.