519051  2,3-dinor Thromboxane B₂ EIA Kit

2,3-dinor Thromboxane B₂ ELISA Kit

You may be eligible to receive a free sample 2,3-dinor Thromboxane B2 EIA Kit under the Cayman Challenge program.

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Limit of detection: 80% B/B0: 7 pg/ml · Sensitivity: 50% B/B0: 35 pg/ml · Thromboxane A2 (TXA2) is produced from arachidonic acid by many cells and causes irreversible platelet aggregation and vascular and bronchial smooth muscle contraction.1,2,3 TXA2 is rapidly hydrolyzed non-enzymatically to form TXB2. Although it is common to estimate TXA2 levels by measuring TXB2, most of the TXB2 measured is due to ex vivo platelet activation or intra-renal production.4 Measurement errors are compounded by the fact that normal concentrations of circulating TXB2 are extremely low (1-2 pg/ml), and highly transient (t½ = 5-7 minutes).5,6 To circumvent this problem, it is necessary to measure a metabolite that cannot be formed by platelets or by the kidney. TXB2 may be metabolized by 11-hydroxy thromboxane dehydrogenase to form 11-dehydro TXB2, or by β-oxidation to form 2,3-dinor TXB2.7 Infusion studies using TXB2 have shown that both metabolites are formed equally, but that the circulating half-life of 2,3-dinor TXB2 is shorter (t½ = 15 minutes). Therefore, measurement of 2,3-dinor TXB2 will give a more episodic indication of TXA2 production.6 Because plasma concentrations of 2,3-dinor TXB2 are low (and artifactual TXB2 concentrations may be high), it may be necessary to purify and concentrate plasma samples. It is also recommended to purify urine samples to remove possible crossreacting metabolites and TXB2 from the sample.8

1  Hamberg, M., Svensson, J., Samuelsson, B. Thromboxanes: A new group of biologically active compounds derived from prostaglandin endoperoxides. Proc Natl Acad Sci USA 72 2994-2998 (1975). 2  Ellis, E.F., Oelz, O., Roberts, L.J., et al. Coronary arterial smooth muscle contraction by a substance released from platelets: Evidence that it is thromboxane A2. Science 193 1135-1137 (1976). 3  Salzman, P.M., Salmon, J.A., Moncada, S. Prostacyclin and thromboxane A2 synthesis by rabbit pulmonary artery. J Pharmacol Exp Ther 215 240-247 (1980). 4  Samuelsson, B., Granström, E., Green, K., et al. Prostaglandins. Annu Rev Biochem 44 669-695 (1975). 5  Patrono, C., Ciabattoni, G., Pugliese, F., et al. Estimated rate of thromboxane secretion into the circulation of normal humans. J Clin Invest 77 590-594 (1986). 6  Lawson, J.A., Patrono, C., Ciabattoni, G., et al. Long-lived enzymatic metabolites of thromboxane B2 in the human circulation. Anal Biochem 155 198-205 (1986). 7  Roberts, L.J., Sweetman, B.J., Oates, J.A. Metabolism of thromboxane B2 in man. J Biol Chem 256(16) 8384-8393 (1981). 8  Lellouche, F., Fradin, A., FitzGerald, G., et al. Enzyme immunoassay measurement of the urinary metabolites of thromboxane A2 and prostacyclin. Prostaglandins 40 297-310 (1990).

	Download Kit Booklet 908 Kb PDF
	Download Abbreviated Kit Protocol 425 Kb PDF
	Download Material Safety Data Sheet (MSDS) 192 Kb PDF

Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.