516521.1  11β-Prostaglandin F2α EIA Kit (Solid Plate)

11β-Prostaglandin F2α ELISA Kit (Solid Plate)



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• See more EIA (Enzyme Immunoassay); Allergy/Asthma

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Limit of detection: 80% B/B0: 5.5 pg/ml · Sensitivity: 50% B/B0: 32 pg/ml · Prostaglandin D2 (PGD2) is the major eicosanoid released from stimulated mast cells. PGD2 is an unstable compound which is rapidly metabolized and eliminated from the circulation. This makes quantitation of PGD2 an unreliable indicator of its in vivo production. 11β-PGF2α is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis.1 The normal human urinary excretion of 11β-PGF2α is about 11 ng/mmol creatinine (~400 ng/24 hr), which is increased nearly 3-fold upon allergen-induced bronchoconstriction in asthmatics.2,3 Unlike most prostaglandin metabolites, 11β-PGF2α retains potent biological activity. 11β-PGF2α is equipotent to PGF2α in inducing human bronchial smooth muscle contractions and inhibition of adipose differentiation.4,5 11β-PGF2α was also shown to inhibit ADP or thrombin-induced human platelet aggregation at concentrations of 0.14 to 2.8 µM.6 The Cayman 11β-PGF2α Assay is a competitive assay that provides accurate measurements of 11β-PGF2α within the range of 7.8-1,000 pg/ml. Inter and intra-assay CV’s of less than 15% can be achieved at most concentrations. This assay allows sensitive detection of 11β-PGF2α in the most common sample matrix, which is urine. Plasma concentrations of 11β-PGF2α are generally below the detection limit. For plasma and other complex sample matrices, we recommend purification of the sample prior to analysis; a purification protocol is included in the kit booklet. Measurements of 11β-PGF2α in urine using our EIA gives values in the same range but slightly higher than those obtained by GC-MS. This appears to be due to measurement of 11β-PGF2α plus the 2,3-dinor metabolite of 11β-PGF2α.2
1  Liston, T.E., Roberts, L.J. Transformation of prostaglandin D2 to 9α,11β-(15S)-trihydroxy-prosta-(5Z,13E)-dien-1-oic acid (9α,11β-prostaglandin F2): A unique biologically active prostaglandin produced enzymatically in vivo in humans. Proc Natl Acad Sci USA 82 6030-6034 (1985).
2  O'Sullivan, S., Mueller, M.J., Dahlén, S., et al. Analyses of prostaglandin D2 metabolites in urine: Comparison between enzyme immunoassay and negative ion chemical ionization gas chromatography-mass spectrometry. Prostaglandins Other Lipid Mediat 57 149-165 (1999).
3  O'Sullivan, S., Dahlén, B., Dahlén, S., et al. Increased urinary excretion of the prostaglandin D2 metabolite 9α,11β-prostaglandin F2 after aspirin challenge supports mast cell activation in aspirin-induced airway obstruction. J Allergy Clin Immunol 98 421-432 (1996).
4  Coleman, R.A., Sheldrick, R.L.G. Prostanoid-induced contraction of human bronchial smooth muscle is mediated by TP-receptors. Br J Pharmacol 96 688-692 (1989).
5  Lepak, N.M., Serrero, G. Inhibition of adipose differentiation by 9α, 11β-prostaglandins F2α Prostaglandins 46, 511-517 (1993).
6  Pugliese, G., Spokas, E.G., Marcinkiewicz, E., et al. Hepatic transformation of prostaglandin D2 to a new prostanoid, 9α,11β-prostaglandin F2, that inhibits platelet aggregation and constricts blood vessels. J Biol Chem 260 14621-14625 (1985).

Download Product Insert 725 Kb PDF
Download Abbreviated Kit Protocol 431 Kb PDF
Download Material Safety Data Sheet (MSDS) 172 Kb PDF

Pricing Information

Prices listed here are the Manufacturer's Suggested European Retail Price. The actual prices quoted by each individual distributor may vary. Please visit the Global Buyer's Guide on the Cayman Chemical (world) website to locate a distributor in your region.

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96 wells €291.00
480 wells €1,090.00
Pricing updated 2010-08-01.
Prices are subject to change without notice.