514010  Prostaglandin E2 EIA Kit - Monoclonal

PGE2 EIA Kit - Monoclonal; Prostaglandin E2 ELISA Kit - Monoclonal



• See below for pricing and ordering
• See more EIA (Enzyme Immunoassay); Assay Kits; Prostaglandins; Immunology; Inflammation

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Limit of detection: 80% B/B0: 15 pg/ml · Sensitivity: 50% B/B0: 50 pg/ml · Prostaglandin E2 (PGE2) is a primary product of arachidonic acid metabolism in many cells. Like most eicosanoids, it does not exist preformed in any cellular reservoir. When cells are activated or exogenous free arachidonate is supplied, PGE2 is synthesized de novo and released into the extracellular space. In vivo, PGE2 is rapidly converted to an inactive metabolite (13,14-dihydro-15-keto PGE2) by the prostaglandin 15-dehydrogenase pathway.1,2 The half-life of PGE2 in the circulatory system is approximately 30 seconds and normal plasma levels are 3-12 pg/ml.3 Our PGE2 EIA has been validated for use with urine, plasma, and culture media samples. In general, urine and culture media samples can be diluted, if necessary, and added directly to the assay well. Plasma samples should be purified prior to use. Because of the rapid metabolism of PGE2, the determination of in vivo PGE2 biosynthesis is often best accomplished by the measurement of PGE2 metabolites. Our Prostaglandin E Metabolite assay (Catalog No. 514531) converts all major PGE2 metabolites into a single stable derivative which is easily measurable by EIA.
1  Granström, E., Hamberg, M., Hansson, G., et al. Chemical instability of 15-keto-13,14-dihydro-PGE2: The reason for low assay reliability. Prostaglandins 19 933-945 (1980).
2  Hamberg, M., Samuelsson, B. On the metabolism of prostaglandins E1 and E2 in man. J Biol Chem 246 6713-6721 (1971).
3  Fitzpatrick, F.A., Aguirre, R., Pike, J.E., et al. The stability of 13,14-dihydro-15 keto-PGE2. Prostaglandins 19 917-931 (1980).

Download Kit Booklet 939 Kb PDF
Download Abbreviated Kit Protocol 420 Kb PDF
Download Material Safety Data Sheet (MSDS) 1032 Kb PDF

Pricing Information

Prices listed here are the Manufacturer's Suggested European Retail Price. The actual prices quoted by each individual distributor may vary. Please visit the Global Buyer's Guide on the Cayman Chemical (world) website to locate a distributor in your region.

This product is also available to buy in bulk quantities. Please contact us for a quote or to purchase.

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Warning This product is not for human or veterinary use.

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Size Price Quantity
96 wells €359.00
480 wells €1,346.00
Pricing updated 2010-08-01.
Prices are subject to change without notice.

Many of our kits require a source of UltraPure Water. If you do not have a source it is available for purchase from Cayman Chemical. Please check the Additional Items Required section of this kit booklet to verify if Ultra Pure Water is needed. Please note that the 480 well kits are supplied with a single 500 dtn vial of tracer and antiserum that must be used within the stated time following reconstitution. Please see the individual kit booklets for these details or contact techserv@caymanchem.com.