10010566  Albumin (bovine) Acetylated

(Ac)BSA (CAS 90488-46-8)

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• See more EIA (Enzyme Immunoassay); Western Blot

For active pharmaceutical ingredients (APIs) related to Albumin (bovine) Acetylated, see Cayman Pharma

Application(s): ELISA and WB · Acetylated BSA may be used as a positive control for ELISA or WB when employing an acetyl-lysine monoclonal or polyclonal antibody. Cayman’s Acetyl Lysine Monoclonal Antibody (clone 7F8) (Catalog No. 10010567) can detect less than 1 ng of this positive control by immunoblotting. Dilutions from a 1 mg/ml stock solution and 2X Laemmli Buffer may be prepared for WB use.1 For example, dissolve the 1 mg lyophilized protein in 1 ml of purified water (aliquot and freeze for later, use as needed), then make ten-fold dilutions until the desired concentration range is obtained. Finally dilute the product 1:1 in 2X Laemmli Buffer. Incubate the prepared sample for five minutes in boiling water followed by five minutes on ice prior to loading gels.Histone subunit modifications such as lysine acetylation are regulated by the activity of histone acetyltransferases (HATs) and histone deacetylases (HDACs).2,3 Epigenetic modifications such as protein acetylation directly influence cellular genetic programs including those contributing to cancer cell viability.4,2,5
1  Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680-685 (1970).
2  Loidl, P. Histone acetylation: Facts and questions. Chromosoma 103 441-449 (1994).
3  Cheung, W.L., Briggs, D.B., Allis, C.D. Acetylation and chromosomal functions. Curr Opin Cell Biol 12 326-333 (2000 Jan 1).
4  Marks, P.A., Breslow, R. Dimethyl sulfoxide to vorinostat: Development of this histone deacetylase inhibitor as an anticancer drug. Nat Biotech 25(1) 84-90 (2007).
5  Bolden, J.E., Peart, M.J., Johnstone, R.W. Anticancer activities of histone deacetylase inhibitors. Nat Rev Drug Discov 5 769-784 (2006).

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Pricing Information

Prices listed here are the Manufacturer's Suggested European Retail Price. The actual prices quoted by each individual distributor may vary. Please visit the Global Buyer's Guide on the Cayman Chemical (world) website to locate a distributor in your region.

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Warning This product is not for human or veterinary use.

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Pricing updated 2010-08-01.
Prices are subject to change without notice.