10009563  sPLA2 (human recombinant Type V)

Secretory Phospholipase A2 (Group V); gVPLA2

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• See more Enzyme Activity; Enzymes; Phospholipase; Proteins; Recombinant Proteins; Inflammation; Metabolism

For active pharmaceutical ingredients (APIs) related to sPLA2 (human recombinant Type V), see Cayman Pharma

Source: human recombinant protein expressed in E. coli · Mr: 14 kDa · Phospholipase A2 (PLA2) catalyzes the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids. PLA2 (Type V) is a secretory PLA2 (sPLA2) of approximately 14 kDa and is one of the isoforms in the growing list of the PLA2 enzyme family.1 This enzyme, rather than the sPLA2 (Type II), is responsible for arachidonic acid mobilization leading to prostaglandin production in macrophages and mast cells.2,3,4 Consistent with this role, sPLA2 (Type V) is associated with the golgi apparatus, nuclear envelope, and plasma membrane in murine bone marrow-derived mast cells.5 sPLA2 (Type V) has been cloned from a variety of species including human, mouse, and rat.6,7,8 The enzyme is expressed in heart, lung, placenta, and spleen, as well as P388D1 macrophages and mast cells.2,3,8 The specific activity of Cayman’s sPLA2 (human recombinant Type V) was established using 1,2-bis(heptanoylthio) Glycerophosphocholine (Cayman catalog no. 62235) as the substrate. The reaction was inhibited by thioetheramide-PC (Cayman catalog no. 62750).
1  Balsinde, J., Winstead, M.V., Dennis, E. Phospholipase A2 regulation of arachidonic acid mobilization. FEBS Lett 531 2-6 (2002).
2  Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A2 involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).
3  Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A2 that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).
4  Bingham, C.O., Murakami, M., Fujishima, H., et al. A heparin-sensitive phospholipase A2 and prostaglandin endoperoxide synthase-2 are functionally linked in the delayed phase of prostaglandin D2 generation in mouse bone marrow-derived mast cells. J Biol Chem 271 25936-25944 (1996).
5  Bingham, C.O., Fijneman, R.J.A., Friend, D.S., et al. Low molecular weight group IIA and group V phospholipase A2 enzymes have different intracellular locations in mouse bone marrow-derived mast cells. J Biol Chem 274 31476-31484 (1999).
6  Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning and recombinant expression of a novel human low molecular weight Ca2+-dependent phospholipase A2. J Biol Chem 269 2365-2368 (1994).
7  Tischfield, J.A., Xia, Y., Shih, D.M., et al. Low-molecular-weight, calcium-dependent phospholipase A2 genes are linked and map to homologous chromosome regions in mouse and human. Genomics 32 328-333 (1996).
8  Chen, J., Engle, S.J., Seilhamer, J.J., et al. Cloning, expression and partial characterization of a novel rat phospholipase A2. Biochim Biophys Acta 1215 115-120 (1994).

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Pricing Information

Prices listed here are the Manufacturer's Suggested European Retail Price. The actual prices quoted by each individual distributor may vary. Please visit the Global Buyer's Guide on the Cayman Chemical (world) website to locate a distributor in your region.

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Size Price Quantity
10 µg €159.00
25 µg €377.00
50 µg €713.00
Pricing updated 2010-08-01.
Prices are subject to change without notice.