10006915  PPARα Transcription Factor Assay Kit

Peroxisome Proliferator-activated Receptor α Transcription Factor Assay Kit



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• See more Transcription Factor Assay; Assay Kits; Nuclear Receptors; PPAR; Atherosclerosis; Endocrinology; Metabolic Syndrome; Metabolism; Obesity

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Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARα, β/δ, and γ, which are expressed at variable levels in different tissues.1 The Cayman Chemical PPARα Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARα binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARα is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
1  Olefsky, J.M. Nuclear receptor minireview series. J Biol Chem 276(40) 36863-36864 (2001).

Download Kit Booklet 533 Kb PDF
Download Material Safety Data Sheet (MSDS) 205 Kb PDF

Pricing Information

Prices listed here are the Manufacturer's Suggested European Retail Price. The actual prices quoted by each individual distributor may vary. Please visit the Global Buyer's Guide on the Cayman Chemical (world) website to locate a distributor in your region.

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96 wells €474.00
Pricing updated 2010-08-01.
Prices are subject to change without notice.